The number of chromosomes of the water buffalois 56 including one pair of sex chromosomes. Males have an X and a Y chromosome whereas females have X and X chromosomes. Sex determination by polymerase chain reaction PCR was found to be a speedy & correct with 95% accuracy at 43.8 days post fertilization increasing to 99.9% at 55.0 days.

If you were asked how you knew you were a male or a female, what would have been your answer? Is it by your looks, by the external genitals? Whatever your answer might be, scientists may not share your view. You may have to get to the laboratory to convince the world that you are either male or female.